Figure 2

Effects of apocynin on cytokine mRNA and protein levels under inflammatory conditions. A, Real time PCR data gathered from mRNA isolated from A549 cells treated with TNF-α (50 ng/ml) in the presence of absence of Apocynin (Apo, 1 mM) for 24 h. Shown are relative levels of inflammatory mediator mRNA in the Apo/TNF-α vs. TNF-α treatment (n = 3; *,p < 0.05; NS, not statistically significant. Cytokine gene expression changes were also measured in BEAS2B cells (B) and PBECs (C), D , Representative raw data from the protein array experiment, comparing cytokine levels in the Apo/TNF-α vs. TNF-α treatment. E, ELISA and densitometeric analysis of cytokine protein array data, comparing cytokine levels in the Apo/TNF-α vs. TNF-α treatment (n = 3; *, p < 0.05; ND, not detected). F, CCL2 expression was measured by ELISA in A549 cells, BEAS2B cells, and PBECs under the treatment conditions described above.