Figure 6

Expression of TLRs in subjects with type 2 diabetes. PBMCs from 10 individuals with diabetes (lean: 2; overweight: 4; obese: 4) were used for total RNA isolation. TLR2/TLR4 mRNA was measured by RT-PCR (A). The RNA expression was normalized to GAPDH housekeeping gene expression. TLRs’ expression is shown in arbitrary units for each group. Results are expressed relative to the expression level in controls and horizontal bars show the mean of each group. The values of expression of TLRs in PBMCs from 12 non diabetic subjects were taken from Figure 1 (B and C).