Figure 5

Expression of IL-6 and TNF-α in PBMCs. The PBMCs from 4 individuals in each group (lean, overweight and obese) were used for total RNA isolation. IL-6 and TNF-α mRNA were measured by RT-PCR (A). The RNA expression was normalized to GAPDH housekeeping gene expression. Cytokines expression is shown in arbitrary units for each group. Results are expressed relative to the expression level in controls and are represented as means ± SE values (B and C). A strong association of TLR2/ TLR4 mRNA with cytokines was observed in PBMCs (D and E). A simple linear regression analysis indicated a significant positive association between adopter protein (MyD88 or IRAK1) and cytokines (F and G).