Table 1 PCR primers and reaction conditions
Gene | Gene accession number | Primers | Product size |
|---|---|---|---|
IL-1β | NM_000576.2 | 5′-AGTGGTGTTCTCCATGTCCT-3′ (sense) | 398 bp |
5′-AGTCAGTTATATCCTGGCCG-3′ (antisense) | |||
IL-8 | NM_000584.3 | 5′-CTTTCAGAGACAGCAGAGCA-3′ (sense) | 510 bp |
5′-CCTACAACAGACCCACACAA-3′ (antisense) | |||
MCP-1 | NM_002981.1 | 5′-CACTCACCTGCTGCTACTCATT-3′ (sense) | 807 bp |
5′-TGTTGAACCAGGATTCACAGAG-3′ (antisense) | |||
MMP-1 | NM_002421.3 | 5′-CCATTCTACTGATATCGGGG-3′ (sense) | 451 bp |
5′-GCCAAAGGAGCTGTAGATGT-3′ (antisense) | |||
MMP-9 | NM_004994.2 | 5′-GAGATTGGGAACCAGCTGTA-3′ (sense) | 569 bp |
5′-TGCAGGATGTCATAGGTCAC-3′ (antisense) | |||
GAPDH | NM_002046.4 | 5′-GACCCCTTCATTGACCTC-3′ (sense) | 360 bp |
5′-GCTAAGCAGTTGGTGGTG-3′ (antisense) |