Figure 2

Differential expression of HLA-DR, CD14, CD16 on monocyte subpopulations in sarcoidosis patients and healthy controls. Median fluorescence intensity (MFI) of HLA-DR (A,B), CD14 (C) and CD16 (D) on classical (CD14⁺⁺CD16⁻), intermediate (CD14⁺⁺CD16⁺) and nonclassical (CD14⁺CD16⁺⁺) monocytes in sarcoidosis patients (n = 38) and in healthy controls (n = 13). Please note that, for sake of clarity, the data in panel A are regrouped in panel B to allow better comparison between the monocyte subpopulations. Values are expressed as mean ± SEM. All three markers were significantly different between the three monocyte subpopulations. Between sarcoidosis patients and healthy controls, nonclassical monocytes showed significant different levels of HLA-DR expression and classical monocytes showed significant different levels of CD14 expression. Comparison of three groups of data (subpopulations of monocytes) was performed by a parametric One-way ANOVA and comparison of two groups of data (patients versus controls) was performed by a two-tailed Student’s t-test.