Figure 1

Neutrophil recruitment into the lung and BAL fluid following allergen challenge. Naïve BALB/c mice were administered a single intratracheal instillation of PBS or GC frass (40 μg/40 μl) and 20 h later, mice were either lavaged to isolate BAL cells or whole lungs were digested and analyzed for neutrophil content by flow cytometry. A. BAL fluid was harvested and differential cell counts performed. Data represent 8 mice per group and are expressed as total cell number mean ± SEM. Statistical significance was determined by ANOVA (*p < 0.001). B. Cells were dissociated from whole lungs and stained for flow cytometry analysis. CD11c + cells were subsequently analyzed for CD11b and Gr1 expression. Data are from a single mouse per group but are representative of 6 mice in each group.