Figure 5

L-NAME-sensitive E-selectin expression and function in vitro. A. Endothelial E-selectin expression in controls (white bar) and 4 hours after treatment with L-NAME (100 μM) or TNFα (10 ng/mL) (black bars). * and ** indicate P < 0.05 and P < 0.01, respectively, difference from the control (t test). Data are means ± SEM (n = 4). B. Rolling neutrophils 4 h after treatment of HUVECs with 100 μM L-NAME (white squares) or 10 ng/ml TNFα (black squares). * indicates P < 0.05 difference from the value at time point 0 min (ANOVA). Data are means ± SEM (n = 4). C. Adhesion of neutrophils 4 h after treatment of HUVECs with 100 μM L-NAME (white squares) or 10 ng/mL TNFα (black squares). * indicates P < 0.05 difference from the value at time point 0 min (ANOVA). Data are means ± SEM (n = 4).