Figure 8

Identification of inflammatory cells in infarcted area. Immunofluorescence stain (A to C) showing the number of CD68+ stained cells (yellow arrows), an indicator of macrophage, markedly increased in AMI than in normal and AMI + tacrolimus, and significantly higher in AMI + tacrolimus than in normal. * vs. other groups, p < 0.0001 (D). Blue color indicated DAPI stain for the nuclei. Immunohistochemical stain (E to G) showing the number of CD40+ stained cells (red arrows), an index of inflammatory cells, substantially higher in AMI than in normal and AMI + tacrolimus, and notably higher in AMI + tacrolimus than in normal. * vs. other groups, p < 0.0001 (H). All statistical analyses using one-way ANOVA, followed by Tukey’s multiple comparison procedure. Symbols (*, †, ‡) indicate significance (at 0.05 level) (n = 6 for each group). HPF = high-power field (400x). The Scale bars in right lower corner represent 20 μm.