Hydrogen saline(20 or 40 μl was added in each well) decreased proinflammation cytokine(TNF-α) concentrations in culture supernatants. Supernatants were collected 4 h after stimulation. TNF-α was measured with an ELISA(7 samples were tested per group).*P < 0.05 compared with LPS+saline group. The murine peritoneal macrophages(A) and the RAW264.7 macrophage cell line(B) had the samilar results. HS = hydrogen saline.