Figure 6

(A) Phagocytic activity of differentiated cells. Cells of the classical pre-plating protocol 2 show significantly higher phagocytosis of fluorescent beads. Cells un-supplemented with ACM and GM-CSF show low phagocytosis. (n = 3). (B) Oxidative burst of the differentiated cells is not different between protocol 1 and protocol 2. P1: Protocol 1. P2: Protocol 2. (n = 3). (C-F) Differentiated cells of protocol 1 are smaller and contain many non-adherent cells. Morphologies and resulting cell types are diverse. Images were taken with a Leica DMIL at 200x magnification. (G-J) Most differentiated cells survive in co-culture and remain largely amoeboid and round, typical for activated states. The cells were co-cultured with living brain slices. Top down and side view pictures. Differentiated cells were labeled green (DIO) and transferred onto 9 day old living brain slices. Dead cells were labeled red (propidium iodide). After 10 days of co-culture a Leica Microsystems SP2 confocal microscope was used to scan the brain slices to a depth of 160 μm (Magnification 100x). (H, J) Cells of the cytokine supplemented cultures invade surface up to a depth of 60 μm. *** = P ≪ 0.001, ** = P ≪ 0.005, * = P ≪ 0.01.