Figure 5
From: Zinc supplementation alters airway inflammation and airway hyperresponsiveness to a common allergen
A20 protein levels following GC frass with or without zinc supplementation. HL-60 cells were treated with GC frass (1 μg/ml) or LPS (1 μg/ml) with or without zinc gluconate (1 μM) and pyrithione (10 μM) for 18 h (A) or for either 2 or 4 h (B). Cell lysates were harvested, separated by gel electrophoresis and probed with an antibody against A20 or β-actin. Each experiment was performed 3 times.