Zinc supplementation decreased NF-κB-DNA binding in whole lung. Mice were given daily i.p. injections of PBS or zinc gluconate (10 mg/kg) for 3 days prior to a single i.t. inhalation of PBS or GC frass (40 μg/40 μl). 4 h later, whole lungs were removed and snap frozen. Lungs were homogenized, nuclear extracts isolated and analyzed by EMSA. NF-κB-DNA binding is shown, top arrows designate supershift of p65 and p50. Data shown are representative from a single mouse (n = 3 mice per group).