Figure 3

A. mRNA for Ub in gastrocnemius muscles measured by semiquantitative RT-PCR normalized to β-actin. Data are means ± SE. Compared to control group, *P < 0.05; to LPS group, #P < 0.05; to low-dose insulin group, $P < 0.05. B. Ubiquitinated protein analysed by western blot with protein levels quantified by densitometry and normalized to β-actin. Compared to control group, *P < 0.05; to LPS group, #P < 0.05; to low-dose insulin group, $P < 0.05.