Figure 2

TMP represses NF-κB dependent reporter activity. The effect of TMP on LPS induced TLR4 signaling was evaluated by reporter analysis. HEK293/TLR4-YFP/MD2 cells were co-transfected with NF-κB -Luc and β-gal control plasmids then, after 4 hours of treatment, luciferase activity was measured in cell lysates (A). To analyze the effects of TMP on other TLR family members HEK293/TLR3 (B) and HEK293/TLR8 (C) cells were co-transfected as above, treated for four hours with 10 μg/ml poly(IC) (B) or 1 μg/ml R-848 (C) and/or 25 μM TMP, and luciferase activity determined in cell lysates. Each experiment was performed at least 3 times and representative experiments are shown. Asterisks indicate significant differences between ligand treatments and ligand treatments with TMP (p < 0.05, T-test).