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Figure 5 | Journal of Inflammation

Figure 5

From: Inflammatory responses in epithelia: endotoxin-induced IL-6 secretion and iNOS/NO production are differentially regulated in mouse mammary epithelial cells

Figure 5

Wedelolactone inhibits ET-induced IL-6 but not iNOS. Total RNA was extracted from SCp2 cells treated with 0 or 10 μM Wedelolactone (W) in DMSO (vehicle) for 1 h before ET treatment at 0 or 10 μg ET/ml for 0, 1, 3, and 6 h. 1 μg total RNA were reverse transcribed then amplified by real-time qPCR for ET induced (A) IL-6 and (B) iNOS mRNA. Amplified mRNA concentrations of IL-6 and iNOS were normalized to those of constitutive GAPDH mRNA. The medium was collected at 1, 3, 6, 12, and 24 h post-ET, and assayed for induced (C) IL-6 secretion and (D) NO production. Controls (DMSO only or Wedelolactone only (not shown)) induced basal levels of both IL-6 and NO production. Data represents the average for duplicate samples ± SD of a representative experiment. (*) denotes significant difference between ET and control groups and @ denotes significant difference between W+ET and ET only treated cells within each time point (p < 0.05).

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Journal of Inflammation

ISSN: 1476-9255

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