Wedelolactone inhibits ET-induced IKK phosphorylation. (A) Nuclear protein of SCp2 cells treated with various doses of the IKKα/IKKβ inhibitor, Wedelolactone (W), were harvested and assayed for phosphorylated and total IκB proteins. Equal loading of proteins was verified by probing for 42 kDa β-actin. (B & C) Densitometric analysis was calculated using NIH Image J program. The intensity of each of the phospho IκB (B) and IκB (C) bands were compared to those of β-actin housekeeping gene expression. Each lane represents one sample at a particular time point. This experiment was repeated two independent times. The same trend of change appeared in both plots, but the blot and densitometric analysis shown represent the stronger change of the two.