Figure 3

Cytokine analysis of macrophages in the presence of activated platelets, apoptotic cells, and dexamethasone. Cytokines were measured by ELISA 24 hrs after stimulation with LPS (100 ng/ml) in the presence or absence of (A) apoptotic cells or platelets, and (B) dexamethasone alone (1 μM) or dexamethasone-loaded platelets. Data for activated platelets is repeated in both panels. Cytokine levels are expressed relative to treatment with LPS only. Macrophages were incubated with equivalent numbers of platelets in each condition. Shown are the averages of at least 3 independent experiments. Statistically significant differences are indicated with corresponding p-values.