Establishing the Acute Model of Murine Asthma. Mice were sensitized with ovalbumin, rested for 14 days and then challenged daily with ovalbumin or sham PBS for 5 days. Mice were sacrificed and the lungs were processed for inflammation with BAL (1A) or histology without BAL (1B saline-challenged (40×) versus 1C ovalbumin-challenged (40×)). Histology is representative of 5 different experiments with 6-8 mice in each group. Inflammation is representative of 5 different experiments with n = 4-6 for each group. Ovalbumin challenged mice had a significant increase in inflammatory cells (n = 4, p = 0.04).