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Figure 1 | Journal of Inflammation

Figure 1

From: Suppression of LPS-induced matrix-metalloproteinase responses in macrophages exposed to phenytoin and its metabolite,5-(p-hydroxyphenyl-), 5-phenylhydantoin

Figure 1

The effect of phenytoin, HPPH and LPS on levels of (A) matrix metalloproteinase-1, (B) matrix metalloproteinase-2, (C) matrix metalloproteinase-3, and (D) matrix metalloproteinase-9 in conditioned medium from macrophage cultures. Primary human monocyte-derived macrophages (n=6 independent cultures) were pretreated with phenytoin or HPPH (15 μg/mL and 50 μg/mL) for 1 hour prior to challenge with 100 ng/mL A. actinomycetemcomitans LPS and the levels of matrix metalloproteinase-1, matrix metalloproteinase-2, matrix metalloproteinase-3, and matrix metalloproteinase-9 measured after 24 hours in conditioned media by multiplex analysis. MMP-1, matrix metalloproteinase-1; MMP-2, matrix metalloproteinase-2; MMP-3, matrix metalloproteinase-3; MMP-9, matrix metalloproteinase-9; CON, control; PHT, phenytoin; HPPH, 5-(4-hydroxyphenyl-),5- phenylhydantoin; LPS, lipopolysaccharide. Compared to CON, # p<0.05, ## p<0.01, ### p<0.001, compared to LPS, * p<0.05, ** p<0.01, *** p<0.001. Student t-test, n=6 independent donors.

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Journal of Inflammation

ISSN: 1476-9255

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