Figure 2

Effect of different concentrations of Tuberosin on LPS induced iNOS expression in attached rat peritoneal macrophages. The macrophages were pretreated with quercetin and tuberosin as given below for 30 minutes and then LPS was added (20 ng/ml) and incubated for 17 hrs. The normal cells were exposed to 0.1% DMSO without any LPS. Lane-1: LPS(20 ng/ml),Lane-2: Normal cells. Lane3:LPS+Quercetine(50 ng/ml), Lane4:LPS+Tuberosine(100 ng/ml), Lane-5: LPS+Tuberosine(300 ng/ml), Lane-6: LPS+Tuberosine(600 ng/ml). The bars depict densitometric analysis of western blot (given in the inset). This picture represents one out of total three experiments carried out separately.