Inhibition of TH1 cytokine secretion by Didox and Trimidox. Briefly, 2 × 105 T-cells per well were purified and seeded in 96 well sterile plates pre-coated with PBS or anti CD3ε antibodies (5 μg/ml). Either PBS or several concentrations of Didox or Trimidox (25 μM- 100 μM) was added to RPMI 1640 growth media supplemented with 10% fetal bovine serum, 2-mercaptoethanol (50 μM) and 1% penicillin streptomycin. The plates were then incubated at 37°C and 5% CO2 for 24 or 48 hours, after which the levels in pg/ml of IFN-γ, IL-2 and IL-6 were determined using the Searchlight multiplex assay system. (A) B10.D2 mice; (B) C57BL6 mice. NC: Normal control, AC: Activated control. The values shown (mean ± SD) represent data obtained in triplicate from two independent experiments. * p < 0.05, ** p < 0.001 indicates a significant difference compared to activated control (AC), ANOVA + the Bonferroni test; n = 3.