Figure 1

Neutrophil and macrophage influx into BAL fluid and lungs of ApoE^-/-mice exposed to CS. Neutrophil and macrophage influx were analyzed in BAL fluid by Diff-Quik staining on cytospin slides (A and B respectively). Data are shown as mean ± SEM (n = 3-4 mice per group). *P < 0.05, ***P < 0.001, significant compared with corresponding air-exposed mice. ⁺P < 0.05, significant compared with CS-exposed WT mice. Lung sections of air- and CS-exposed WT and ApoE^-/- mice were stained with anti-mouse Mac-3 antibody (C). Mac-3-positive cells (dark brown) were identified by immunohistochemical staining (indicated by arrows and insets), Original magnification: ×200. Histogram (D) represents mean ± SEM. **P < 0.01, significant compared with corresponding air-exposed mice. ⁺⁺P < 0.01, significant compared with CS-exposed WT mice. ^##P < 0.01, significant compared with air-exposed WT mice (n = 4).