Figure 1

Qa-2 expression in PBMC of BD. A. RT-PCR demonstrated that mRNA expression was lower in PBMC of BD skin than in BD normal mice. B. The frequency of Qa-2 in PBMC of normal healthy controls, BD asymptomatic (BD normal) mice, BD mucocutaneous symptomatic mice (BD skin), and BD mucocutaneous and ocular symptomatic mice (BD eye) as determined by FACS analysis. In lymphocytes, the Qa-2 levels in BD eye mice were significantly lower than in normal healthy mice (p = 0.036). These levels were also lower than in BD skin mice, although this difference was not significant. In granulocytes, the Qa-2 levels in BD eye mice were significantly lower than in normal healthy mice (p = 0.016). The Qa-2 levels in BD eye mice were lower than in normal and BD skin mice, although this difference was not statistically significant. Qa-2 levels in BD skin were significantly lower than in normal controls (p = 0.024). C. The portion of Qa-2 positive cells in lymphocytes or granulocytes. The frequency of Qa-2 positive cells in the granulocytes of BD skin and BD eye mice was lower than in normal controls and BD normal mice (BDN). The frequencies of Qa-2 positive cells in BD eye mice were significantly lower than those in normal controls (p = 0.001).