Figure 6

Effect of silencing Pyk2 expression on chemotaxis of dHL-60 cells to fMLP. A) Representative Western blot of Pyk2 expression in dHL60 cells (top panel). dHL-60 cells were resuspended in nucleofector solution V (V), and then transfected with 2 μM Pyk2 siRNA (sipyk2) or non-targeting scramble control siRNA (scram). Pyk2 expression in dHL60 cells was measured 3 days after transfection by Western blotting analysis using anti-Pyk2 antibody, and equal protein loading was verified by anti-β actin antibody. The bottom panel was the densitometric analysis of Pyk2 bands from three blots. **P < 0.01 vs. nucleofector solution V vehicle control or scramble control using paired t test. B). Effect of silencing Pyk2 expression on migration of dHL60 cells. Migration assays were performed 72 h after transfection on Transwell plates as above. Migration in response to 10^-8 M fMLP was measured after 90 min. Results are presented as the mean ± SEM from 3 separate experiments. *P < 0.05 vs. nucleofector solution V vehicle control or scramble control using paired t test.