Cutaneous dermal burn injury induces a significant local inflammatory response as evidenced by soluble mediator production. Chemokine, proinflammatory cytokine and PGE2 synthase gene transcript levels at the wound edge/margin were assessed using quantitative RT-PCR as described in the Material and Methods. Representative results from two independents experiments are shown. The expression of transcripts normalized to 18 s at the wound margin at days 1, 3 and 7 post thermal injury were determined. Each value represents the mean ± SEM fold increase in gene expression versus naïve uninjured skin from control MRL/MpJ mice. (n = 10 mice/time point, all data points have P values < 0.05 as compared with naïve skin).