Figure 16

Western Blot analysis of Bax and Bcl-2 expression. Representative Western Blot of Bax and Bcl-2 levels was realized in pulmonary samples at 18 h after zymosan-injection. At 18 h after zymosan administration, a significant increase in the Bax expression was observed in the lung (A, see densitometric analysis A1) compared to the sham-treated mice, whereas in GW0742-treated mice Bax levels were substantially reduce (A, see densitometric analysis A1). On the contrary, at 18 h after zymosan administration, a decrease in the Bcl-2 expression was observed in the lung (B, see densitometric analysis B1) compared to the sham-treated mice, while Bcl-2 expression was more evident in the pulmonary tissue from zymosan-treated mice that received GW0742 treatment (B, see densitometric analysis B). Data are mean ± standard deviation from n = 10 mice for each group. *P < 0.01 vs sham, °P < 0.01 vs zymosan + vehicle.