Figure 11

Effect of GW0742 on peritoneal exudates (A) and plasma nitrate/nitrite levels (B). Nitrate/nitrite levels were significantly increased both in peritoneal exudate and in plasma of zymosan-treated mice in comparison to vehicle group (sham group). GW0742 reduced the zymosan-induced increase of nitrate/nitrite levels in peritoneal exudate (A) and in plasma (B). In addition, by Western Blot analysis the iNOS expression was evaluated in the lung. At 18 h after zymosan administration a significant increase in the iNOS expression was observed in the ileum (Ca, see densitometric analysis a1) compared to the sham-treated mice. GW0742 treatment significantly reduced the iNOS expression (Ca, see densitometric analysis a1). The intensity of bands was measured using a phosphoimager in all the experimental groups. β-actin was used as internal control. A representative blot of lysates obtained from each group is shown, and densitometric analysis of all animals is reported (n = 5 mice from each group). Data are mean ± standard deviation from n = 10 mice for each group. *P < 0.01 vs sham, °P < 0.01 vs zymosan + vehicle.