Regulation of TLR2 induction peptidoglycan by fatty acids and pharmacological inhibitors of ERK (p44/42 MAPK), JNK and NFκB. In cells pretreated with 500 μM linoleic acid (LIN), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and then treated with peptidoglycan (6A), TLR2 mRNA expression was further additively upregulated in the presence of the fatty acids and peptidoglycan. Additionally, in cells were pretreated with the specific inhibitors, 10 μM U0126 and SP600125 (SP) and 50 μg/ml SN50 before peptidoglycan treatment for 6 hours. Inhibition of ERK and JNK, but not NFκB (6B), additively upregulated peptidoglycan induced TLR2 expression. Bars represent means and ± SEM of this normalized expression from 4 different replicates. Bars with different superscripts are different (P < 0.05).