Regulation of TLR2 and TLR4 mRNA expression by LPS, peptidoglycan and linoleic acid. Expression of both TLR2 (5A) and TLR4 (5B) was determined in cells treated with LPS (100 ng/ml), peptidoglycan (10 μg/ml) and linoleic (500 μM) acid for 6 hours. Significant induction of TLR2 (5A) (approx.7 fold) and TLR4 (5B) (1.5 times) was obtained. Bars represent means and ± SEM of this normalized expression from 4 different replicates. Bars with different superscripts are different (P < 0.05).