Lipopolysaccharide (LPS) and peptidoglycan (PEP) induce IL6 mRNA expression and protein secretion. Cells were treated with 100 ng/ml LPS and 10 μg/ml peptidoglycan (PEP) for 3, 6 and 24 hours. Media and mRNA were recovered for each time period and assayed for IL6 gene expression using RT-PCR (1A) and protein secretion with ELISA (1B). mRNA and media concentration of IL6 were increased by LPS and PEP. Bars represent means and ± SEM of this normalized expression from 4 different replicates. Bars with different superscripts are different (P < 0.05).