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Figure 1 | Journal of Inflammation

Figure 1

From: Stimulation with Peptidoglycan induces interleukin 6 and TLR2 expression and a concomitant downregulation of expression of adiponectin receptors 1 and 2 in 3T3-L1 adipocytes

Figure 1

Lipopolysaccharide (LPS) and peptidoglycan (PEP) induce IL6 mRNA expression and protein secretion. Cells were treated with 100 ng/ml LPS and 10 μg/ml peptidoglycan (PEP) for 3, 6 and 24 hours. Media and mRNA were recovered for each time period and assayed for IL6 gene expression using RT-PCR (1A) and protein secretion with ELISA (1B). mRNA and media concentration of IL6 were increased by LPS and PEP. Bars represent means and ± SEM of this normalized expression from 4 different replicates. Bars with different superscripts are different (P < 0.05).

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Journal of Inflammation

ISSN: 1476-9255

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