Figure 3

Thrombin stimulates angiogenic growth hormone secretion predominately from primary cultures of newly differentiated human adipocytes. Cultures of adipose tissue (n = 5), newly differentiated adipocytes (n = 5) or preadipocytes (n = 3) were stimulated with thrombin (1 U/ml) or thrombin (10 U/ml) in the presence or absence of a thrombin inhibitor (lepirudin) for 24 hours. PBS served as the negative control. The following growth factors were measured from the supernatants of the cultures. A) FGF-2; B) PDGF and; C) VEGF. Means ± SEM *P < 0.05 thrombin vs. PBS. ± P < 0.05 thrombin + lepirudin vs. thrombin.