Figure 1

PAR1 and PAR4 are present in adipose tissue. A) PAR1 mRNA amplified from cDNA from adipose tissue from 5 individuals (lanes 1–5). The positive control (lane 6) contains cDNA from a human artery and the negative control (lane 7) contains PAR1 specific primers but no cDNA. B) PAR4 mRNA amplified from cDNA from adipose tissue from 5 individuals (lanes 1–5). The positive control (lane 6) contains cDNA from a human artery and the negative control (lane 7) contains PAR4 specific primers but no cDNA. C) Representative immunoblots of tissue or cells from 2 individuals (A and B). PAR1 and PAR4 protein detected in adipose tissue (lanes 2 and 4) and adipose microvessels (lanes 3 and 4) but only PAR4 is detected in Preadipocytes (lanes 7 and 9) and differentiated adipocytes (lanes 8 and 10). Lane 1 is a positive control lysate. Lane 6 contains the molecular weight marker. A total of 6 groups (whole tissue and isolated preadipocytes and differentiated adipocytes) were analyzed for PAR1 and PAR4.