Figure 6

The pharmacodynamic effect of MK0812 on monocytes is mediated by CXCR4. A, Blood and bone marrow were isolated from naïve BALB/c mice and stained for expression of CD11b, Ly6G, Ly6C and CXCR4. Neutrophils were excluded by gating on the Ly6G^- population and monocytes were gated upon based on CD11b⁺Ly6C^hi expression. The expression of CXCR4 on the gated CD11b⁺Ly6C^hi monocytes is indicated. B, BALB/c mice were administered the indicated dosage of vehicle (MC) or AMD3100 s.c. 1 h later, mice were dosed p.o. with MK0812 or vehicle. 30 min later, peripheral blood CD11b⁺Ly6C^hi monocyte numbers were determined as in Figure 2. Plasma concentrations of CCL2 and CXCL12 were determined from the same mice. ***p < 0.001, *p < 0.05 compared to the Vehicle group. C, The effect of AMD3100 was assessed on Ly6G^-CD11b⁺Ly6C^hi monocytes and Ly6G⁺Ly6C⁺ neutrophils. **p < 0.01 as shown. The data presented are the mean ± S.E.M. of six mice per group and are representative of three independent experiments.