Figure 2

Effect of RIAA on the inhibition of GSK3 pathway. (A) GSK-3α was incubated with RIAA (0, 5, 25 and 50 μg/ml) and the kinase activity was determined. Data represent 1 representative experiment and are expressed as % activity. (B) GSK-3β was incubated with RIAA (0, 1, 5, 25 and 50 μg/ml) and the kinase activity was determined. Data represent 1 representative experiment and are expressed as % activity. (C) RAW 264.7 cells were incubated with RIAA (0, 1, 5, 10 and 20 μg/ml), or GSK-3 inhibitors LiCl (10 mM) and SB216763 (10 μM) for 1 h. Cell lysates were analyzed for the inhibition of phosphorylation of β-catenin by western blot.