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Figure 5 | Journal of Inflammation

Figure 5

From: CCR5 signalling, but not DARC or D6 regulatory, chemokine receptors are targeted by herpesvirus U83A chemokine which delays receptor internalisation via diversion to a caveolin-linked pathway

Figure 5

U83A delays internalisation of CCR5 and effects on localisation of caveolin-1. Localisation of CCR5 and caveolin-1 were monitored by confocal microscopy in the absence of chemokine stimulation, buffer only (a, d, g), and when stimulated for 10 minutes with CCL4 (b, e, h) or U83A (c, f, i). DAPI nucleus staining was used as a control, which gave nuclear staining of all cells indicated (not shown). After stimulation, cells were fixed and permeabilised, then reacted with CCR5 antibody linked to FITC, green channel (a, b, c) showing internalisation induced by CCL4 (b), and caveolin-1 antibody linked with TRITC, red channel (d, e, f), showing coalescence of punctuate staining induced by U83A (f). The merged staining (g, h, i) shows for U83A treatment, examples of punctae of caveosome-like structure, with yellow merged fluorescence as indicated in the enlarged insets (i). Representative 0.39 micron slices are from the z-stack from three independent assays.

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Journal of Inflammation

ISSN: 1476-9255

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