Effect of PIN1 knockdown on COX-2 mRNA. A, KD and Control (Con) shRNA MAEC were treated with LPS and IFN for 0–8 h. B, Cells were treated with medium or LPS and IFN for 24 h. mRNAs encoding COX-2 and β-actin were determined by RT-PCR and agarose gel electrophoresis. Representative ethidium bromide-stained gels are shown. Replicate COX-2 or β-actin products in a single gel were imaged for analysis. Bars represent mean + S.E. ratio of COX-2: β-actin products from densitometric analysis of images from 3 independent cultures. *:p < 0.05 for comparison with cells treated for 0 h in A, or with medium in B.