Figure 6

The effects of TMP on copy number of cytokine mRNA. RAW 264.7 cells were left untreated (Media), or treated with LPS (1 μg/ml) and/or TMP (25 μM). After 16 h, total RNA was extracted from the cell pellet and mRNA copy number determined by q-RT-PCR for TNF-α (A), MCP-1(B) and RANTES (C). Panels show means ± SEM of representative experiments with duplicate copy number determinations performed in each experiment. Asterisks indicate significant differences between treatments with LPS and LPS/TMP (p < 0.05, Student's t-test).