Figure 3

The effects of TMP on cytokine production. RAW 264.7 supernatants were collected and assayed for cytokine production using the Mouse Cytokine Array I (RayBiotech, Norcross GA). Shown in this figure are scans of films developed from array filters following incubation with supernatants from either untreated control cells (A), or from cells following incubation with 25 μM TMP (B), 1 μg/ml LPS (C), or 25 μM TMP and 1 μg/ml LPS (D). All supernatants were collected following a 16 h incubation period. The cytokines, chemokines, growth factors, and inflammatory products detected by the array and their respective coordinates are: Eotaxin, H1/2; Eotaxin-2, I1/2, Fas Ligand, J1/2; Fractalkine, K1/2; GCSF, L1/2; GM-CSF, A3/4; IFN-γ, B3/4; IL-1α, C3/4; Il-1β, D3/4; IL-2, E3/4; IL-3, F3/4; IL-4, G3/4; IL-6, H3/4; IL-9, I3/4; IL-10, J3/4; IL-12p40p70, K3/4; IL-12p70, L3/4; IL-13, A5/6; IL-17; B5/6; I-TAC, C5/6; KC, D5/6; Leptin, E5/6; LIX, F5/6; Lymphotactin, G5/6; MCP-1, H5/6; M-CSF, I5/6; MIG, J5/6; MIP-1α, K5/6; MIP-1γ, L5/6; RANTES, A7/8; SDF-1, B7/8; TCA-3, C7/8; TECK, D7/8; TIMP-1, E7/8; TIMP-2, F7/8; TNF-α, G7/8, sTNF R1, H7/8; sTNF R2, I7/8. Positive controls are located at positions A1, B1, C1, D1, K8, and L8. Negative controls are located at positions A2, B2, C2, and D2. Blanks are located at positions E1, E2, J7, J8, K7, and L7. Solid and dashed ellipses indicate the coordinates of cytokines and chemokines induced by LPS to high and low levels, respectively, as discussed in the text.