Figure 3

SDS-PAGE and Gelatin zymography represent the expression of MMP-2. Detection, by gelatin zymography, of matrix metalloproteinase-2 (MMP-2) and (MMP-9) expression at different time points after the induction of Cadmium (5 mg/Kg body weight). Lung cell extracts were prepared at days of 15, 30, 45 and 60 from normal (N), Cadmium treated and Cadmium plus Ibuprofen treated (three individual animals per dose) mice. The zymography was developed and stained as described in Materials and Methods. The picture shows increased expression of MMP-2(72 kD), which was not inhibited by Ibuprofen. Gel is representative of three comparable experiments indicate p < 0.05 with respect to the control.