Figure 5
From: Inhibition of zymosan-induced kidney dysfunction by tyrphostin AG-490
Effect of AG-490 on plasma C5a and zymosan-specific IgG antibody levels, on C5aR expression in kidneys and on the IgGs binding to glomeruli. A, AG-490 diminished the level of circulating C5a on day 21 post-zymosan injection. AG-490 inhibited C5aR expression in kidneys. Infiltrating cells in glomeruli, Ba and tubular epithelial cells, Bb of shock kidneys were positive for C5aR staining (arrows). Bc, d, The renal C5aR expression was undetectable in AG-490-treated mice; 1 × 400; *p < 0.05 versus mice with shock, Student's t test. C, Zymosan injection induced anti-zymosan IgG antibodies production that was significantly inhibited by AG-490; **p < 0.01 versus zymosan injected group. The results are expressed as mean ± SD of 5 mice per group from 2 experiments. D, The representative data from 2 experiments showed the glomerular binding of IgGs isolated from zymosan-immunized mice (b, d, ZY-positive serum) but not of IgGs isolated from healthy mice (a, c; normal serum). Arrows showed the areas of positive staining.