Figure 5
From: Cigarette smoke regulates the expression of TLR4 and IL-8 production by human macrophages
CSM induces generation of ROS in MDMs. MDMs were pretreated with NAC (10 mM) for 30 min and then stimulated with CSM (0.03 and 0.06 OD) for 1 h. Intracellular ROS concentration was measured by incubation of cells with H2DCFDA as a probe for 30 min at 37 o C. Then after washing, the density of flurochrom as indicator for generation of ROS was determined by FACS analysis. The results were expressed as fold increase over control cells. Data represent means ± SEM of triplicate experiments (n = 3). * p < 0.05 versus unstimulated control; # p < 0.05 versus CSM.