Figure 3
From: Cellular and molecular mechanisms of cigarette smoke-induced lung damage and prevention by vitamin C
A, Immunoblots of caspase 3, PARP, Bax and Bcl-2 of the lung extracts of guinea pigs exposed to air or CS supplemented with 1 mg or 15 mg vitamin C/animal/day. Vit C means vitamin C. Air + vit C means exposed to air and fed 1 mg or 15 mg vitamin C/day; CS + vit C, means exposed to CS and fed 1 mg or 15 mg vitamin C/day. B, quantitative evaluation of Bax/Bcl-2 ratio. C, detection of apoptosis in the lung of guinea pig fed 1 mg vitamin C and exposed to CS for 21 days by immunofluorescence analysis using cleaved caspase 3 antibodies; lower panel I, stained with TRITC (red signal); II, stained with DAPI (blue); III, double staining with DAPI and TRITC; IV, enlarged version of III. D, colocalization of cleaved caspase 3 (CC3) in lungs of CS-exposed guinea pigs fed 1 mg vitamin C; red signal (TRITC) indicates cleaved caspase 3; blue signal, DAPI and green signal, TUNEL. Merged mens combination of TRITC, DAPI and TUNEL; image captured by laser scanning confocal microscope; original magnification × 63. E, Accumulation of Bax proteins in the lungs of CS-exposed guinea pigs fed 1 mg vitamin C/day; red signal, stained with TRITC and blue signal, stained with DAPI. Merged means combination of TRITC and DAPI.