Figure 4

LysoPC treatment of normal rats. Twelve rats were treated with subcutaneous injections of 0.25 mg/kg lysoPC in PBS containing 8% ethanol or PBS-ETOH vehicle only. The rats experienced mild restraint during the injections. Two hours later, the rats were sacrificed and plasma sPLA2 activity was measured by determining the amount of pyrenyldecanoic acid (PDA) generated from the 10-pyrene substrate in one hour. A typical spread of enzyme activities was seen in the vehicle-treated rats while lysoPC treatment produced a range of activities that were significantly narrower and lower. * = p < 0.05, Welch test.