Figure 7
Post treatment of 15d-PGJ2 inhibits monocyte adhesion to activated endothelial cells. bEND.3 cells were treated with TNFα (50 ng/ml) for 6 h, followed by addition of different concentrations of 15d-PGJ2 (5–20 μM). After 30 min of incubation with 15-PGJ2, fluorescently labeled monocytes were allowed to interact with activated bEND.3 cells. Adhered monocytes were counted as mentioned in 'Material and Methods'. Data calculated as mean ± SD of 21 fields from 3 different experiments. *** p < 0.001 compared to untreated control cells and @ p < 0.001 compared to TNFα treated cells.