Modulation of LPS stimulated NF-kappaB mediated Nitric Oxide production by PKCε and JAK2 in RAW macrophages

Journal of Inflammation

Table 1

	Nitrite (μM); mean ± SEM
	Control	Concentration of peptide alone		1 μg/ml LPS with stated concentration of peptides below
		10 μM	100 μM	0 μM	1 μM	10 μM	100 μM
MALY O1	0.70	2.00	0.77	44.6	46.1	47.2	51.6
	± 0.2	± 1.6	± 0.3	± 2.7	± 4.2	± 5.1	± 2.0
MALY 1O	0.70	0.87	0.85	44.6	47.3	40.3	46.4
	± 0.2	± 0.4	± 0.3	± 3.3	± 0.8	± 1.9	± 0.9
MALY II	0.70	0.64	0.66	44.6	46.2	43.4	45.9
	± 0.2	± 0.3	± 0.3	± 2.8	± 0.9	± 2.9	± 0.5

RAW cells were stimulated with medium alone (control), LPS (1 μg/ml) or various concentrations of the MALY TAT-linked PKC translocation inhibitor peptides (MALYO1 = cPKC inhibitor, MALY1O = PKC specific, MALYII = scrambled PKC inhibitor peptide), with or without LPS for 24 hr. NO production was assessed by Greiss assay. Results represent mean ± SEM (n= 3) and are representative of two separate experiments.

ISSN: 1476-9255