Figure 3

NF-κB activation in LPS and PMA stimulated RAW 264.7 cells. RAW cells were stimulated with LPS (1 μg/ml) and PMA (50 ng/ml) for between 0–120 minutes. Cells were harvested and nuclear NF-κB-p65 levels were assessed by Western blotting. (a) Representative Western blot of p65 expression. Equal amounts of nuclear proteins were loaded onto each lane. (b) Graphical representation of % increase in p65 nuclear localisation shown in (a) above. Data is presented as mean of 2 independent experiments.