Figure 1

a. The effect of PMA on LPS stimulated NO production in RAW 264.7 cells. RAW cells were stimulated for 24 hr with either vehicle alone (control), 1 μg/ml LPS, 50 ng/ml PMA or 1 μg/ml LPS with 50 ng/ml PMA. NO levels were assessed by Greiss assay. LPS alone significantly increased NO production whereas PMA alone had no effect. PMA significantly inhibited LPS -stimulated NO production. Figure 1b (inset) displays the concentration-response curve for LPS-stimulated NO production over 24 hrs. Results are expressed as mean ± SEM; ⁺⁺⁺p < 0.001 vs control, ***p < 0.001 vs LPs-stimulated; n = 6 for the effects of PMA and n = 9 for the concentration-response curve.