Figure 4

Cartilage explant production of IGF-1 and the effects of IL-1β and progrado. Control, untreated human cartilage explants release a defined amount of IGF-1 into the bathing media. Cartilage release of IGF-1 was significantly reduced by IL-1β (10 ng/ml, * P < 0.05). Co-treatment with progrado 2 or 10 μg/ml restored and raised IGF-1 production above basal controls (** P < 0.01). This effect was more pronounced with the lower dose of progrado (2 μg/ml) than the higher dose (10 μg/ml). In the absence of IL-1β, progrado (10 μg/ml) reduced IGF-1 production basal values (* P < 0.01). Results are depicted as the mean ± sem.