| |
Final concentration (mol/l)
|
Activity of lipoxygenase
|
% Inhibition
|
IC50 (mol/L)
|
|---|
|
Aqueous solution of jatrorrhizine (0.01M)
| | | | |
| |
15.10-6
|
118.71 ± 4.37
|
22.05 ± 1.37
|
17.50.10-6 ± 1.27.10-6
|
| |
20.10-6
|
23.89 ± 1.83
|
84.32 ± 3.74*
| |
| |
25.10-6
|
12.73 ± 1.24
|
91.58 ± 2.91*
| |
|
Control
|
-
|
152.29 ± 5.37
|
-
| |
|
Aqueous solution of berberine (0.01M)
| | | | |
| |
15.10-6
|
233.19 ± 7.41
|
14.88 ± 0.97
|
30.50.10-6 ± 2.87.10-6
|
| |
20.10-6
|
213.40 ± 6.93
|
22.11± 1.24
| |
| |
25.10-6
|
157.02 ± 4.21
|
42.69 ± 2.38
| |
|
Control
|
-
|
273.96 ± 8.21
|
-
| |
- Lipoxygenase activity was determined as absorbance increase at λmax = 234 nm at 3 minutes of incubation with or without inhibitor tested. Values of hydroperoxide content and lipoxygenase activity were calculated from equation c = A.V/ε.l.v, where A is the value of absorbance increase, V is the volume of incubation mixture, ε is the extinction coefficient for linolic acid (25.10-3 mol.l.cm-1), l is the length of the cuvette (1 cm) and v is the volume of enzyme (0.015 ml). Results are presented as percent of control ± SD, n = 3, * p < 0.05 vs. controls
