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Table 2 Results of lipoxygenase inhibitory effects of two representative alkaloids isolated from Mahonia aq. crude extract.

From: Free radical scavenging activity and lipoxygenase inhibition of Mahonia aquifolium extract and isoquinoline alkaloids

  Final concentration (mol/l) Activity of lipoxygenase % Inhibition IC50 (mol/L)
Aqueous solution of jatrorrhizine (0.01M)     
  15.10-6 118.71 ± 4.37 22.05 ± 1.37 17.50.10-6 ± 1.27.10-6
  20.10-6 23.89 ± 1.83 84.32 ± 3.74*  
  25.10-6 12.73 ± 1.24 91.58 ± 2.91*  
Control - 152.29 ± 5.37 -  
Aqueous solution of berberine (0.01M)     
  15.10-6 233.19 ± 7.41 14.88 ± 0.97 30.50.10-6 ± 2.87.10-6
  20.10-6 213.40 ± 6.93 22.11± 1.24  
  25.10-6 157.02 ± 4.21 42.69 ± 2.38  
Control - 273.96 ± 8.21 -  
  1. Lipoxygenase activity was determined as absorbance increase at λmax = 234 nm at 3 minutes of incubation with or without inhibitor tested. Values of hydroperoxide content and lipoxygenase activity were calculated from equation c = A.V/ε.l.v, where A is the value of absorbance increase, V is the volume of incubation mixture, ε is the extinction coefficient for linolic acid (25.10-3 mol.l.cm-1), l is the length of the cuvette (1 cm) and v is the volume of enzyme (0.015 ml). Results are presented as percent of control ± SD, n = 3, * p < 0.05 vs. controls