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Table 2 Results of lipoxygenase inhibitory effects of two representative alkaloids isolated from Mahonia aq. crude extract.

From: Free radical scavenging activity and lipoxygenase inhibition of Mahonia aquifolium extract and isoquinoline alkaloids

 

Final concentration (mol/l)

Activity of lipoxygenase

% Inhibition

IC50 (mol/L)

Aqueous solution of jatrorrhizine (0.01M)

    
 

15.10-6

118.71 ± 4.37

22.05 ± 1.37

17.50.10-6 ± 1.27.10-6

 

20.10-6

23.89 ± 1.83

84.32 ± 3.74*

 
 

25.10-6

12.73 ± 1.24

91.58 ± 2.91*

 

Control

-

152.29 ± 5.37

-

 

Aqueous solution of berberine (0.01M)

    
 

15.10-6

233.19 ± 7.41

14.88 ± 0.97

30.50.10-6 ± 2.87.10-6

 

20.10-6

213.40 ± 6.93

22.11± 1.24

 
 

25.10-6

157.02 ± 4.21

42.69 ± 2.38

 

Control

-

273.96 ± 8.21

-

 
  1. Lipoxygenase activity was determined as absorbance increase at λmax = 234 nm at 3 minutes of incubation with or without inhibitor tested. Values of hydroperoxide content and lipoxygenase activity were calculated from equation c = A.V/ε.l.v, where A is the value of absorbance increase, V is the volume of incubation mixture, ε is the extinction coefficient for linolic acid (25.10-3 mol.l.cm-1), l is the length of the cuvette (1 cm) and v is the volume of enzyme (0.015 ml). Results are presented as percent of control ± SD, n = 3, * p < 0.05 vs. controls
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Journal of Inflammation

ISSN: 1476-9255

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