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Table 2 Perfusion effects on the concentration of analyte.

From: Demonstration of a novel technique to quantitatively assess inflammatory mediators and cells in rat knee joints

 

Animal 1

Animal 2

 

IL1β concentration (pg ml-1; 250 μl)

Amount of IL1β (pg)

IL1β concentration (pg ml-1; 250 μl)

Amount of IL1β (pg)

1 hour

2000

500

2000

500

2 hour

2000

500

2000

500

3 hour

200

50

356

89

4 hour

544

136

216

54

5 hour

350

87.5

210

52.5

6 hour

458

114.5

200

50

7 hour

318

79.5

  

Total (pg)

1467.5

1245.5

  1. IL1β concentrations in each 250 μl sample collected, up to 7 hours post-infusion of IL1β (1000 pg). The amount of IL1β protein in each sample was calculated and summed, to show that little or no leakage from the joint space occurred. In fact, more IL1β was present than was injected, in both cases as a result of de novo release of endogenous IL1β protein.
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Journal of Inflammation

ISSN: 1476-9255

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